Our mission of AACL is to continueously develop novel diagnostic and therapeutic products for immunological diseases in animals by using advanced technology of allergy and immunology, finally contributing to progress veterinary science. Since AACL was established as RIKEN venture company, we would like to fully utilize technology and knowledge on allergy and immunology for veterinary fields.

1. Allergy test for the dog

<Antigen-specific IgE quantitative assay>

The quantitative measurement of IgE, patented by AACL and RIKEN (Patent number Japan:5334269 USA:8, 716, 031 Australia:2009247267), is utilized by veterinarians to compare serum IgE concentrations among allergens, resulting in their identification of the most offending allergen in dogs suffred from allergic diseases. By monitoring serum IgE concentration, veterinarians are able to understand a change of causative allergens over different seasons.

・Related references

Okayama T, Matsuno Y, Yasuda N, et al. Establishment of a quantitative ELISA for the measurement of allergen-specific IgE in dogs using anti-IgE antibody cross-reactive to mouse and dog IgE. Vet Immunol Immunopathol 2011;139:99-106.

Ichikawa Y, Beugnet F. Epidemiological survey of anti-flea IgE in dogs in Japan by using an antigen-specific IgE quantitative measurement method. Parasite 2012; 19:173-6.

<Lymphocyte proliferative response assay>

Proliferated T-helper lymphocytes against food allergens are measured by flow cytometoric technology. Percentages of CD4+CD25+ cells were shown in this assays.

・Related references

Masuda K, Yasuda N. The antibody against human CD25, ACT-1, recognizes canine T-lymphocytes in the G2/M and G0/G1 phases of the cell cycle during proliferation. J Vet Med Sci 2008;70:1285-1287.

Fujimura M, Masuda K, Hayashiya M, et al. Flow cytometric analysis of lymphocyte proliferative responses to food allergens in dogs with food allergy. J Vet Med Sci 2011;73:1309-1317.

Kawano K, Oumi K, Ashida Y, Horiuchi Y, Mizuno T. The prevalence of dogs with lymphocyte proliferative responses to food allergens in canine allergic dermatitis. Pol J Vet Sci 2013; 16:735-9

Suto A, Suto Y, Onohara N, et al. Food allergens inducing a lymphocyte-mediated immunological reaction in canine atopic-like dermatitis. J Vet Med Sci 2014.

<CCR4/CD4 positive lymphocyte assay>

This is to measure peripheral T-helper lymphocytes positive for CCR4 molecule. The cells are known to increase in peripheral blood in dogs with atopic dermatitis. Flow cytometry is used for this assasy.

・Related references

Yasuda N, Masuda K, Maeda S. CC chemokine receptor 4-positive CD4(+) lymphocytes in peripheral blood increases during maturation in healthy beagles. J Vet Med Sci 2008;70:989-992.

Yamaya Y, Watari T. Increased proportions of CCR4(+) cells among peripheral blood CD4(+) cells and serum levels of allergen-specific IgE antibody in canine chronic rhinitis and bronchitis. J Vet Med Sci 2015; 77:421-5

2. Elimination diets

LaboLine, PureProtein series are elimination diets for food allergy in dogs, containing a single protein. There are 3 products; PureProtein Wheat, PureProtein Chichen, PureProtein Salmon.

Development of novel vaccines especially focusing on zoonosis under collaboration with RIKEN

Development of feline allergy testing services.